Journal: Acta biomaterialia

Article Title: In vivo short-term and long-term host reaction to starch-based scaffolds.

doi: 10.1016/j.actbio.2010.06.020

Figure Lengend Snippet: Fig. 2. Micrographs of the sections of the explanted FB and WS produced starch-based scaffolds after 1 (A–D) and 2 (E–H) weeks of IM implantation in rats. Tissue was immunohistochemically labelled for CD18 and CD3. represents the SPCL scaffold fibres, arrows mark CD18-positive cells and dashed arrows mark CD3-positive cells.

Article Snippet: The histological analysis of crosssection samples was performed after haematoxylin and eosin (HE), and Masson Goldner Trichrome (MGT) staining and immunohistochemistry using a monoclonal mouse anti-human CD3 antibody (Dako, Denmark) with cross-reactivity for rat T lymphocytes, a monoclonal mouse anti-rat CD18 antibody (Serotec, UK) for the integrin b2 chain of recruited leukocytes, and a monoclonal mouse anti-human phosphoinositide 3-Kinase (Pi3K) antibody (BD, Belgium) with cross-reactivity for rat activated and proliferating lymphocytes, following standard protocols.

Techniques: Produced, Starch

Journal: Acta biomaterialia

Article Title: In vivo short-term and long-term host reaction to starch-based scaffolds.

doi: 10.1016/j.actbio.2010.06.020

Figure Lengend Snippet: Fig. 5. Micrographs of the sections of the explanted FB and WS produced starch-based scaffolds after 1 (A–D) and 2 (E–H) weeks of SC implantation in rats. Tissue was immunohistochemically labelled for CD18 and CD3. represents the SPCL scaffold fibres, arrows mark CD18-positive cells and dashed arrows mark CD3-positive cells.

Article Snippet: The histological analysis of crosssection samples was performed after haematoxylin and eosin (HE), and Masson Goldner Trichrome (MGT) staining and immunohistochemistry using a monoclonal mouse anti-human CD3 antibody (Dako, Denmark) with cross-reactivity for rat T lymphocytes, a monoclonal mouse anti-rat CD18 antibody (Serotec, UK) for the integrin b2 chain of recruited leukocytes, and a monoclonal mouse anti-human phosphoinositide 3-Kinase (Pi3K) antibody (BD, Belgium) with cross-reactivity for rat activated and proliferating lymphocytes, following standard protocols.

Techniques: Produced, Starch

Journal: BMC Physiology

Article Title: The tripeptide feG ameliorates systemic inflammatory responses to rat intestinal anaphylaxis

doi:

Figure Lengend Snippet: Activation markers on interstitial cells. Effects of feG on staining of interstitial leukocytes in the jejunal muscle for CD18 and ED9 antigens.

Article Snippet: The intestinal tissues were incubated for 24 h at 4°C with either ED9 or CD18 primary antibody (Cedarlane, Hornby, ON) at a 1:100 dilution) followed by three 5-min washes in PBS.

Techniques: Activation Assay, Staining, Control

Journal:

Article Title: Leukocyte-Mediated Endothelial Cell Injury and Death in the Diabetic Retina

doi:

Figure Lengend Snippet: CD18 blockade prevents leukocyte adhesion and endothelial cell injury and death in diabetic retinal venules and capillaries. A: After treatment with anti-CD-18 F(ab′)2, diabetic retinal leukocyte adhesion was markedly decreased in the retinal venules and capillaries. B: Analysis with PI revealed a concomitant decrease in retinal endothelial cell injury and death; however, no change in arteriolar endothelial cell injury and death was observed.

Article Snippet: In separate experiments, diabetic rats received either 1 mg/kg of a mouse anti-rat CD18 F(ab′) 2 (WT.3) (Associates of Cape Cod Inc., Falmouth, MA) or a purified mouse anti-human non-immune F(ab′) 2 (Jackson ImmunoResearch Laboratories Inc., West Grove, PA).

Techniques:

Journal:

Article Title: Leukocyte-Mediated Endothelial Cell Injury and Death in the Diabetic Retina

doi:

Figure Lengend Snippet: Anti-CD18 surface staining of a static leukocyte within a vessel. Green fluorescence from the FITC-linked Concanavalin A (A) and red fluorescence from the anti-CD18 antibody (B) identify the leukocytes as being CD18-positive when the images are superimposed (C).

Article Snippet: In separate experiments, diabetic rats received either 1 mg/kg of a mouse anti-rat CD18 F(ab′) 2 (WT.3) (Associates of Cape Cod Inc., Falmouth, MA) or a purified mouse anti-human non-immune F(ab′) 2 (Jackson ImmunoResearch Laboratories Inc., West Grove, PA).

Techniques: Staining, Fluorescence